Using Apple's Compressor and Flip4Mac. I am applying deinterlacing since it is originating from DV video for use on the web. My best settings were:
Video: 2-pass VBR at a bit rate of 236kbps, peak bit rate 472, 320x180, 29.97fps, Windows Media 9 Standard, Quality slider set to 0 for smoothest playback, Complexity control set at "Offline Slow", Main profile, keyframe every 5 seconds, buffer delay set to 5 seconds, B-Frame distance at zero frames, input: interlace - lower field first, output: progressive.
Audio: 1-pass CBR at 64kbps, 44.1khz, stereo.
Client must have 300 kbps data rate or under.
MAK Digital Media, Inc.
Video Post-Production Services
Orlando, FL