Yea, try re-encoding the asset if you haven't. It sounds like an issue with the media you're using.
You can also try a new project if you haven't already (just a simple one to see if it works OK to create a new project). Your current project may just be corrupt for some reason. Might have to rebuild it. I've never actually had that happen, though.
And FYI for future projects, you should always choose Build in DVDSP when you're ready to produce your DVD. After the build, choose Format. Always separate. And never use Burn.
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